PLEASE CONSULT US BEFORE STARTING YOUR PROTEOMICS EXPERIMENT
Read carefully:
1. Basic guidelines – UH Proteomics Core
2. Sample preparation guidelines for protein identification from a gel
3. Potential interference and contaminant ions in MS
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Below you can find proteomic protocols that may be useful for your experiment.
– Protein sample preparation for nano-LC/MS
– In-gel digestion protocol for MALDI-MS
– In-solution trypsin digestion
– Nano-LC with ion-trap analysis
– Peptide mass fingerprint by MALDI workflow
– Preparation of cryostat sections for MALDI imaging
– Data Analysis – maXis Impact