TRMD 690 Seminar:”Development of Microfluidics for Use in a Novel Way to Characterize Latently HIV-Infected Cells”, Bradley Mak, MS Student

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Date(s) - 01/26/2022
12:00 PM - 1:00 PM


Zoom Meeting ID: 979 6297 5142

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Bradley Mak

Tropical Medicine MS Student

Department of Tropical Medicine, Medical Microbiology and Pharmacology
John A. Burns School of Medicine, University of Hawaii at Manoa

Microfluidics has numerous applications in the biomedical field. We aim to develop a low-cost, microfluidics-based approach (termed HIV-Seek) to isolate, identify and characterize latently HIV-infected cells, which remain poorly understood despite being a major barrier to HIV cure. The main challenges to characterization of this viral reservoir are 1) latently infected cells are rare (frequency of 1/106 resting memory CD4+ T-cells) and 2) activation is required to determine latency but also rewires gene expression. Custom microfluidics allows us to perform low-cost experiments to overcome these challenges. In this presentation, I will describe the fabrication and basic function of microfluidic devices for use in HIV-Seek. Additionally, I will be discussing our progress in integrating microfluidics and molecular biology to develop our novel methodology.