The Histology & Imaging Core at Kaka’ako is supported by a generous grant from the Research Centers in Minority Institutions, and by the Center for Cardiovascular Research and the Department of Cell and Molecular Biology. It provides routine histology processing, specialized histochemical and immunohistological techniques, and access to several microscopic platforms. The core also provides technical assistance, training, and consultation in histological and imaging techniques to investigators throughout the University on a cost-recovery basis.

Services:

* Histology processing, including paraffin embedding, frozen or       floating sections, staining and other conventional histology       techniques

* Training and advice for general histology & imaging techniques

* Development of new immunohistochemical or in situ hybridization approaches as needed

Facilities and Equipment:

The histology core is located on the second floor of the Kaka’ako campus of the University of Hawai’i. Based on funding provided by both COBRE and RCMI grants, the core includes a Zeiss LSM 5 Pascal Confocal Microscope, fluorescent and optical scopes, robotic tissue processing, a variety of microtomes and a cryostat.

How to use the core:

To reserve time on the equipment, please sign up on worksheets located in the core. Potential users must demonstrate competence with the equipment before use. Training can be scheduled with Qingping He. All users must complete a user form and the charge sheet located in the core.

To submit histology work, please fill out the work request database form (provided on a computer in the histology room).

To submit paraffin or cryosections for immunocytochemistry or in situ hybridization, antibody or probe information needs to be included on the histology work request form. Primary antibody or probe should be provided by PIs. The core provides all general supplies except special items requested for special projects.