Plant Propagation

TPSS 420


Hye-Ji Kim

Semesters taught:

Spring 2014, Spring 2015, Spring 2016


Propagate plants by seed, cuttings, grafts, and layers and understand the basis for pathogen-free and aseptic propagation. Recognize appropriate practices of sanitation, disease control, and use of rooting hormones. Appreciate the importance and value of record-keeping in the nursery operation and be familiar with the major components of the cost of production of propagules.

Skills and knowledge to be acquired:

As a result of study and training in this course, the student is expected to be able to: 1. Understand and explain physiological aspects of propagation by means of seeds, cuttings, grafts, and layers. 2. Choose among various propagation systems and techniques for a variety of plant materials to effect efficient and successful plant increase.

Computer skills to be acquired:



TPSS 200 or consent


Plant Propagation Principles and Practices (6th Edition), H. T. Hartmann, D. E. Kester, F.T. Davies, Jr., and R. L. Geneve. 1997. Prentice Hall. Upper Saddle River, NJ.


Practical Woody Plant Propagation for Nursery Growers. B. Macdonald 1986. Timber Press, Portland, OR.

Films and videos:

Grafting Apple Trees, RMI Media Productions; Budding: New Life for Fruit & Nut Trees, Vocational Educational Prod.; Grafting Methods for Propagating Ornamentals, Robert L. Fincham; Introduction to Plant Tissue Culture, Creative Educational Video, Inc.; Automated plug growing, Infinite Video; Bedding Plant Production, VEP Video Productions

Guest Speakers:

Frank Sekiya, Stan Oka

Field Trips:

City & County of Honolulu Kapiolani Nursery; Frankie's Nursery (Waimanalo)

Course organization:

  • Introduction, Record-keeping, Planning Propagation
  • Structures, Nursery layout
  • Media & Containers
  • Environment of plant propagation
  • Physiology of vegetative propagation
  • Stock plant management
  • Techniques of Propagation by cuttings
  • Disease prevention & control
  • Budding & Grafting - Theory & Practice
  • Aseptic culture
  • Seed Development
  • Collection, Handling & Storage of Seed
  • Seed dormancy
  • Seed germination
  • Seed treatments
  • Seedling production systems
  • Plug culture
  • Cost of Production


20% for each of 2 midterms, 20% for Final Exam, 30% for laboratory notebook, separate lab writeups, and homework; 10% for student presentation.